Shanghai cell scratches experiment.

Shanghai cell scratches experiment. Simple and easy to detect cell movement methods can be used to detect invasive metastasis capabilities of the wall growth tumor cells.

Shanghai cell scratches experiment. Experimental content:

Material: 6-well plate (other can also, but feel 6 holes is better, small), Marker pen, straight, 20 Steps to be sterilized by microlifting gun head (sterilization), serum medium, PBS

. MARKER pen, ruler is irradiated before the operation of 30 min (over the Tower)

1. First use the Marker pen behind the 6-well plate, with a straight comparison, evenly spread the horizontal line, approximately every 0.5-1cm, cross the hole, passing at least 5 lines per well.

2. In the air, about 5 * 105 cells are added, and the specific quantity is different due to cells, and the mastery is full of liquid can be full

3. The next day with the gun head is straight. The ruler is perpendicular to the horizontal scratches behind it. The gun head is vertical and cannot be tilted

4. Use PBS to wash cells 3 times, remove the cells, and serum-free medium.

5. Put 37 degree 5% CO2 incubator, cultured, according to 0.6. 12 24 hours sampling, taking a photo.

Precautions:

Generally scratch experiment, generally cultured in serum-free or low-serum state, otherwise cell proliferation cannot be ignored [123

According to the vertical scratch of the 6-well plate, a number of intersections can be formed as a fixed monitoring point, which solves the problem of not being fixed in the position of the position before and after.