Shanghai miRNA sequencing experiment

Shanghai miRNA sequencing experiment

miRNA sequencing is a known miRNA in a particular state to analyze new miRNA, and target gene predictions known miRNA and new miRNA. . By analyzing miRNA target gene notes and metabolic pathway analysis, identified miRNA is associated with corresponding phenotypes, providing a powerful tool for studying the role of cell processes and their biological impacts.

Shanghai miRNA sequencing experimental biological information analysis content

1, data output statistics and quality assessment

2, SRNA distribution on the reference genome

3, SRNA classification annotation statistics

4, alkali preference analysis

5, gene expression level analysis

6, new transcriptional prediction

7 , Differential miRNA target gene prediction

8, differential gene screening and clustering

9, differentially expressing miRNA target gene GO enrichment analysis

10, differentially expressing miRNA target Gene pathway enrichment analysis

11, differentially expressing miRNA target gene protein mutual network analysis

Technical parameters

Sample type: Total RNA

Sample requirements: Total u0026 ge; 3 u0026 Mu; g, concentration u0026 ge; 200 Ng / u0026 Mu; L

Library Type: Small RNA Library

Sequencing Policy: Hiseq SE50 , Recommended data volume: u0026 ge; 10m reads

Project cycle: 35 working days

Sample

Sample known miRNA all position Base preference

Novel miRNA secondary structure and reads mapping schematic

Various samples There is a total of Novel miRNA distribution