Shanghai Acer

1. Service introduction
Acer is not required to separate purification of each microorganism, but directly from Sequencing analysis was performed after extracting genome DNA in the sample. Through the macro-based group sequencing, the microbial group structure, evolutionary relationship, functional activity and the environment can be explained, and the microbiology research range is expanded. Environmental microbial diversity detection refers to high-throughput sequencing by PCR amplification products in the environment in the environment, analyzes the diversity and distribution law of microbial community under this environment. Second, operational flow

1. Genomic DNA extraction
2. DNA random interrupt
u0026 nbsp ; 3. Add a sequencing street, build a library
4. Sequencing
5. Sequence stitching
u0026 nbsp ; 6. Bioinformatics analysis

1. Species identification
2. Diversity statistics Analysis
4. Functional analysis
5. Microbial community structure and function
[123 ] Four, Shanghai Acer Due Sequencing Experiment
Sample Requirements 1. Sample Type: The genome DNA (Double-strand DNA sequence) of human microorganisms.
2. The amount of sample: Construct small fragment library, the total amount of DNA sample is not less than 5 u0026 mu; g.
3. Sample quality: OD260 / 280 value Between 1.8 ~ 2.0; the main band of the sample should be above 23 kB, and the genome has no degradation. 4. Sample concentration: The higher the concentration of the sample, the better, low should not be less than 50 ng / u0026 mu; l.
5. Send sample requirements: send The sample is good to keep a low temperature environment, and the method is to freeze the post, followed by dissolving TE, of course, can also be dissolved in double vapor, and the sample indicates the solvent component.
V. Quality Commitment Provide reliable data and analysis results

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