Transcription group sequencing experiment

Transcription group sequencing experiment Product introduction

Transcription group sequencing ( Transcriptome sequencing is a transcriptional and gene sequences that quickly obtain a certain species specific cell or tissue in a certain state through the second-generation sequencing platform, which can be used to study gene expression, gene function, structure, variable splicing and new Transcription This prediction, etc. At present, transcription group sequencing has been widely used in basic research, clinical diagnosis and drug research and development. Shanghai AY uses standardized experimental processes, advanced sequencing platforms and professional information analysis, with high efficiency, high-quality technology services to win the good reputation in industry professionals.

Second,

Operation process

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2. MRNA separation and library construction;

[ 123] 3. High-throughput sequencing;

4. Data processing and biological information analysis;

[ 123] 5. Sequencing report generation

Third,

(1) Transcription group without reference genomic sequence

[ 123]

1) Remove the processes and low quality reads for raw data;

2) The components and quality assessments of data output statistics and sequencing data; 3) Gene expression quantitative analysis

4) Analysis of assembly results;

5) UniGene Function notes and COG analysis; GO classification; Pathway analysis; NR analysis; Swissprot analysis [ 6) UNIGENE expression difference analysis;

7) Expression mode cluster analysis of different genes [ 8) GO function enrichment analysis and Pathway enrichment analysis of differentially expressing genes;

10) SNP analysis

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Transcription regulatory network analysis, and network map construction and important TF analysis;

[ 123] Circular interactive network analysis, important path analysis.

Differentially expression gene co-expression analysis, constructing a network map Transcription factor activity analysis , Predict important transcription factors;

Gene common upstream regulation element prediction

miRNA It is predicted that important miRNA

[123

1) Treatment of the original data to remove joint pollution sequence and low quality READS

2) Reference genomic ratio

3) Performing a quantitative analysis

4) variable shearing analysis (eukaryotic)

5) New transcript this prediction: new mRNA and new LNCRNA prediction

6) Differential expression gene analysis (2 or more samples) 7) Expression mode cluster analysis of differential genes

8) GO function of different genes Analysis and Kegg Pathway enrichment analysis 9) Protein interaction network analysis of differential genes

[123 10) SNP analysis

2. Analysis [ 123]

Transcription regulatory network analysis, and network diagram construction and important TF analysis; Circular interactive network analysis, important path analysis.

Differentially expression gene co-expression analysis, constructing a network map

Transcription factor activity analysis , Predict important transcription factors; Gene common upstream regulation element prediction

miRNA It is predicted that the important miRNA

drug is small Molecular prediction analysis (suitable for humans), resulting in pharmaceutical small molecules.

4, sample requirements

1) Sample type: cell, fresh tissue or RNA sample.

2) The amount of sample: Cell sample provides at least 1 u0026 Times; 107 cells, tissue samples, provide at least 300 mg tissue block or slice, RNA sample please Total RNA above G or more. 3) Sample quality: RNA has no obvious degradation, the extraction total RNA OD260 / 280 value is between 1.8 to 2.2, concentration u0026 ge; 500 ng / u0026 mu ; L, 28S: 18S u0026 ge; 1.5, rIN u0026 Ge; 8.

4) Samples Save: Cell samples or fresh tissue blocks (cut to 50 mg small pieces) can be treated with Trizol or RNA protective agent or liquid nitrogenAfter, -80 ° C is stored.RNA samples are soluble in ultrapure water of ethanol or RNA-Free, and -80 ° C is stored.During the storage period, avoid repeated freeze-thaw.

5) Sample Transport: Samples are placed in 1.5 ml tubes, sealed membranes are sealed, dry ice transport.

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