Fluorescence quantitative PCR experiment
Fluorescent quantitative PCR detection technology has been born to date has been more than 10 years The time, and its application has not been widely launched, the reason is not subject to the development of related instruments, reagents and technologies. Recently, especially in 2008, the instruments and reagents have flown everywhere, which also enables researchers to try, and I want to take this technology to make my own research can be flew, and the development momentum can be found by finding the number of articles each year. According to statistics, in the Medline database, use u0026 ldquo; Taqman u0026 rdquo; or u0026 rdquo; Real Time PCR u0026 RDQUO; as a keyword retrieval, 1996 is 19, 157 in 1999, in 2003, as high as 2,984, how much is the 2009 Woolen cloth? We don't know. However, its rapid development momentum is not changeable. The company sincerely hopes to work together with many researchers to seize this good time, and contribute their strength for the development of scientific research. Based on this goal, the company has long been studied in-depth research on fluorescent quantitative PCR, whether technology or years of products, and launch more excellent fluorescence quantitative PCR technical services in time. Second,
Fluorescence quantitative PCR experimentservice Process
1, primer design and synthesis 2, DNA / RNA extraction
3, reverse transcription (for RNA)
4, the machine is quantified Analysis Third,
Customer provides
1, whole blood, tissue or cell. 2, primers, or supplied.
3, gene information: purpose gene name, species and sequence (or genbank accession number). Fourth, delivery content
Original data: Dissolved graph, amplification graph, CT value