Steady strain screening experiment

Steady strain screening experiment

Steady transformation cell line (stabilizing expression cell line) refers to the continuous stability expression of a particular gene or interfere with specific gene expression in cells. The purpose of steady cell strain is integrated into the cell chromosome, allowing the cells to express the gene long term. The steady transmissal cell strain includes a polyclonal stable transform strain and a monoclonal radiocellular strain. After the cells infected with a slow viral infection, the cell line obtained by the resistant gene screen is a combination of multiple cells. The monoclonal cell line was selected from the cell cloning from the polyclonal cells, and the obtained cell line was amplified by a cell. Unity of monoclonal cell lines, the gene expression of cells in the biochemical process remains stable. The blunt transient transfection and the method of stabilizing cell lines have rich experience in instantaneous transfection and through a slow viral system. It can do it for difficulty transfected cells such as NB4 cells. Down to 50% transfection efficiency.
Trichry Screen Screening Experiment Service Process

Gene / Template DNA Synthesis

Vector Construction u0026 NBSP

Slow virus package Harvested virus Q-PCR detection of viral titer Slow virus steady transcetum strain screening [ 123] You should provide details of the purpose of genetic information and carriers in accordance with experiments.

Viral titer detection report; steady transcendelling strain;

, including detailed experimental processes, Sequencing results and peak maps, primer sequences, carrier maps, and titer measurement reports and QPCR stable transitions.

Service content: 1, stable: 15 generations stabilized transfection cell stable expression.

2, rapid: General control over 1 month Complete the build within.

3, economy: price affordable, cost-effective.

4, quality: Strict identification of exogenous genes.