Expressing lentiviral construction and packaging experiment

over-expressing lentiviral packaging construct and test

over-expressing lentivirus product, the customer to transfer the gene sequence of the CDS lentiviral vectors, The lentiviral particles of the transaction target gene were obtained by packaging;

Lentivirus (LV) belongs to the reverse transcription viral department, which is an RNA virus. The transformed slow virus is used as an exogenous gene carrier with its unique features and advantages. As a special retroviral vector, a special retroviral vector has a large amount of fractional cell and non-division cells, the transfer gene fragment is large, and the purpose of gene expression is long, which is not easily induced by the host immune response. It has become a current gene therapy vector. Research on the hotspots of research.

The slow viral carrier has the following advantages: (1) * Transfection efficiency: transfection of genes can be transfected into a living mode organism and mammalian Cells, including split and non-divided cells. The non-split cells are in a state in which the growth is not active or stopped, and it is very difficult to transfection, such as neuronal cells, primary cells, stem cells, etc., which are close to the transfection efficiency of such cells.
(2) Stable and long-term expression: the carrier has sequence elements with high efficiency packaging, transfection and stabilize the genome of the target cells, and the genetic recombination of the gene. To form a stable genetic substance, the target gene can be stable in cells. (3) High security, low immunogenicity: viral carrier itself inactivation, no virus replication. The slow virus expression vector provided by Shanghai World Hirt is self-replicating inactivation. Since the cells do not have a viral housing gene, transfected cells do not produce viral particles. In addition, the integration of the genome after the genome, the 5 u0026 RSquo; -LTR promoter is inactive, preventing the copy of the slow virus.
(4) Controlled expression: Controllable expression has always been an important direction of carrier research, and the slow viral expression vector modified by a particular element can achieve only available exogenous or endogenous. It can be expressed under the action of matter to regulate the timing and quantitative expression of the target gene.


Overexpression of slow virus construction and packaging experiment
: Various reporters (GFP, RFP, MCHERRY, Luciferase, EGFP-Luciferase, etc.) or slow virus, puromycin, zeocin, purified virus titer can reach 1 u0026 Times ; 108 - 1 u0026 Times1010 TU / mL, can meet the amount of viral quantity required for the cellular level and the need for different specification viral amplification titers.Technical services include: overexpression of lentiviral construction and packaging, RNA interference lentiviral construction and packaging, miRNA overexpress lentiviral construction and packaging, miRNA closed leapfrot virus construction and packaging.