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Shanghai ordinary electrophoresis PCR experiment

Time:2018-09-03 Click:1212

Electrophoresis is a commonly used laboratory technology that can be identified , Quantitatively and purify nucleic acid fragments. The sample is placed on the hole of the agarose or acrylamide gel and placed in an electric field to move the negatively charged nucleic acid. The shorter DNA fragment migrates faster, and long fragments will be close to the starting point, thereby achieving separation based on DNA fragment size.


General electrophoresis PCR: agarose gel electrophoresis, through gel electrophoresis technology, and the detection method of a fragment of a DNA produced. This method is convenient and simple.

The purpose of the separation nucleic acid molecule and detecting nucleic acid molecules were reached under the action of the molecular sieve by a suitable gel medium. Phenol blue and xylene are generally used as an indicator. When the experiment is generally, it is necessary to pay attention to the temperature of the tap, and the production of the glue needs to pay attention to the direction of the sieve pull out, the buffer cannot be too many.

Shanghai General Electrophoresis PCR Experiment Service Content: Detection of 1 DNA fragment.

ENBSP; Purification of the fragment of 2 DNA.

3, analysis and statistics of experimental data. ;