Gene chip experiment
Using the principle of hybridization, that is, DNA according to the principle of the base, at normal temperature. In conjunction with neutral conditions, a double-stranded DNA molecule is formed, but under conditions such as high temperature, alkaline or organic solvent, the hydrogen bond between the double helix is u200b u200bbroken, double spiral, form a single-strand molecule (referred to as DNA denaturation, DNA denaturation Temperature is called TM value). The denatured DNA viscosity decreases, the settlement speed is increased, the buoyancy has increased, and the ultraviolet absorption increases. When the denaturation conditions are eliminated, the denaturation DNA has two complementary strands to restore the original double helix structure, which is called replication. After reproaching DNA, its physicochemical properties can be restored.
With DNA's important physical and chemical characteristics, the polynucleotide chains of more than two different sources are complementarily caused by complementarity. The process of forming heterogeneous molecules is called hybridization. The molecules in the hybrid are not from the same dimer molecule. Since the temperature is more easily controlled than other denaturing methods, when the double-stranded nucleic acid is higher than its denaturation temperature (TM value), the spiral is unconnected to a single-stranded molecule; when the temperature is lowered below the Tm value, the single-stranded molecule according to the base The pairing principle is again resilient into a double-stranded molecule. It is therefore usually used to change the DNA in the process of denaturation and restructuring.
Data analysis (an example of miRNA chip analysis)
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Chip pretreatment and standardization; [123 Screening difference expression microRNA;
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Targeting the predicted microorna target gene for GO enrichment analysis pathway Enrichment analysis, etc.
Personalized analysis
[123 ] 1.MicrorNA network analysis: MicroNA target gene offset network; Micronra function synergistic network.[123 2. Implement analysis of microRNA and other chip: Cerna analyysis; integrated analysis with mRNA chip; methylation with DNAJoint analysis of chip results.
3. Analysis with clinical data