ELISA Experimental Technical Services
ELISA Experimental Technical Services
ELISA is based on antigen or antibodies Solidization and enzyme label of antigen or antibodies. An antigen or antibody binding to the surface of the solid-phase carrier remains its immunological activity, the enzyme-labeled antigen or antibody retains its immunological activity, and reserved the activity of the enzyme. At the time of assay, the detected specimen (the antibody or antigen in which the antibody or antigen) reacted with the antigen or antibody surface of the solid phase carrier. The antigenic antibody complex formed on the solid phase carrier is separated from the other substances formed in the liquid using the washing method. The enzyme-labeled antigen or antibody is added, and it is also bonded to the solid support by the reaction. The amount of enzyme on the solid phase is in the quantity of the subject in the specimen. After the substrate of the enzyme reaction, the substrate was catalyzed into a colored product, and the amount of the product was directly related to the amount of the subject in the specimen, so it can be qualitatively or quantitatively analyzed according to the color of the color. Since the catalytic efficiency of the enzyme is high, the result of the immune response is indirectly enlarged, and the measurement method reaches high sensitivity.
ELISA Experimental Technical Services Note: There is a complete plan before collecting specimens, clear whether the ingredients to be detected is sufficiently stable. We advocate fresh specimens to detect as soon as possible, the specimens of testing after collecting, timely store at 4 ° C spare, if there is a special reason to collect specimens, please build the specimen, place the specimen, put it in -20 ° C Or prepare under -70 ° C. Due to the temperature difference between the ice chamber and the room temperature, the protein is easy to degrade, directly affects the quality of the experiment, so avoid repeated freeze-thaw. On behalf of the appraisal, the customer should be submitted to our sales staff to explain, please communicate with our technical personnel.
Liquid specimens include serum, plasma, urine, thoracicum water, cerebrospinal fluid, and cell culture supernatant. Serum: After 10-20 minutes, it was centrifuged for about 20 minutes (2000-3000 rp / min). Carefully collect the supernatant. If there is a precipitate during storage, it should be centrifuged again.
Plasma: EDTA, sodium citrate or heparin should be selected as anticoagulant according to the requirements of the specimen, and after 10-20 minutes, centrifugation is about 20 minutes ( 2000-3000 rpm). Carefully collect the supernatant. If there is a precipitate during storage, it should be centrifuged again. Urine: Collection with sterile tubes. Centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. If there is a precipitate during storage, it should be centrifuged again. Chest and abdomenWater, cerebrospinal fluid refers to this.
Cell culture supernatant: When the secretion is detected, collected with a sterile tube.Centrifuge for about 20 minutes (2000-3000 rpm).Carefully collect the supernatant.When the intracellular ingredient was detected, the cell suspension was diluted with PBS (pH 7.2-7.4), and the cell concentration reached 1 million / ml.The cellular ingredients were destroyed by repeatedly freezing.Centrifuge for about 20 minutes (2000-3000 rpm).Carefully collect the supernatant.If there is a precipitate during storage, it should be centrifuged again.
Tissue specimens: After cutting the specimen, weigh the weight.Add amount PBS, pH 7.4.Quickly freeze with liquid nitrogen.The temperature of 2-8 ° C was maintained after the specimen was melted.The added amount of PBS (pH 7.4), which is sufficiently spirally tie with the manual or homogenizer.Centrifuge for about 20 minutes (2000-3000 rpm).Carefully collect the supernatant.After the installation, the remaining freeze spare.