JRD0071 Morphology HE Service

Morphology HE Service

Morphological HE Service

: The conventional tissue slice is substantially colorless transparent, and it is not easy to observe under the general light microscope, so it is necessary to have different structures according to the organization. Specificity to choose different experimental methods. Sumu u0026 mdash; Eros, referred to as HE staining method, common dyeing method in pathological technology. Suichum refinement is alkaline, making the cells to nuclear purple blue; Inghong is acidic, so that the cells are red, HE staining is to use a wide range of technical methods in histological, pathological studies, to observe the basic structure of the organization. .

The entire dyeing process includes five contents: dewaxing, dyeing, dehydration, transparent and sealing.

(1) Dewaxing: 1. Remove the grilled sliced u200b u200bfrom the warm box, immediately insertion of xylene 5 to 10 min (can be carried out in the second bottle), the dewaxing time is low depending on the wax is insoluble. The temperature is low in temperature, and the temperature can be shortened by high temperature or the degree off wax in the warm box. 2. Transfer to the waterless alcohol () (2 bottles), about 2 min.
3. Move into 90% alcohol (two bottles), about 2 min.
4. Move into 80% alcohol (two bottles), about 2 min.
5. Transfer to 70% alcohol, about 2 min.
6. Move into the water, wash away alcohol, about 2 to 3 min.
7. Move into distilled water, about 2 min.
(2) Dyeing:
1. Move into Sukin, soaked 8 to 15 min, it is generally slightly induced.
2. Move into the water, wash it into Suimin and floating, about 1 to 2 minutes.
3. Transfer to differentiated fluids (1% hydrochloride alcohol), differentiation for a few seconds to 30 seconds, making the slice faded to the divertes. The role of differentiation can make the cell pulp to take off, and the nucleus is clearer, fresh, indifferent, and the cytosollan, nuclear nuclear is over-dyed, and the nucleus is too dimming, it is difficult to defend it. Sukin dyeing solution, extended time.
4. Movered into the water, washed 30 ~ 60min, so that the tissue is fresh or blue (Lanhua).
5. Movered into Iraqi, dip 2 ~ 5min, such as dyeing, can add glacial acetic acid (100 ml of Iran with 1 ~ 2 drops of ice acetic acid) in Iraq.
6. Move into the water, wash away the red floating liquid, and wipe the excess dye on the slide with gauze.
(3) Dehydration:
1. After the moisture on the slide, after 80% alcohol, (two bottles) dehydrated, about 1 ~ 2min, if faded in alcohol, can be quickly moved into 90 % Alcohol or return to Iraqi solution.
2. Move into 90% alcohol (two bottles) dehydration, about 2 to 4 minutes.
3. Transfer to waterless alcohol (alcohol) (two bottles) dehydration, about 4 to 8 minutes.
(4) Transparent
1. Transparent 3 to 5 min in xylene I.
2. Transparent 5 to 10 min in xylene II.
(5) Sealing:
Sealed with gum, first removes the slice from the xylene II, rapidly rubbing the xylene peripheral, dripping a drop of gum on tissue sheet, then take a clean coverThe slide is carefully added to the greaser, slowly flattened, so that the cover is moderate.After the slice is sealed, it is placed in the warm box, or places the cool drying box.


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