JRD284EMSA Homology Technical Service

EMSA Hydrology Technical Services

is a gel migration or electrophoretic mobility experiment (EMSA) is a study of DNA binding proteins and related The technology of DNA binding sequence interactions can be used for qualitative and quantitative analysis. This technique is initially used to study DNA binding proteins, which have been used to study the interaction of RNA binding proteins and specific RNA sequences. Purified proteins and cell coarse extracts and 32P isotope labeled DNA or RNA probes are typically thermally insulated, and the complexes and non-binding probes are separated on non-denathetic polypropylene gel electrophoresis. DNA-composites or RNA-composites are slower than non-binding probes. The isotope labeled probe is different from the binding protein of the study, but the double-strand or a single strand. When the DNA binding protein such as transcriptional regulators is detected, a purified protein, a partially purified protein, or a nuclear cytokinetic liquid can be used. When the RNA binding protein is detected, the protein can be extracted or partially purified according to the position of the RNA binding protein. DNA or RNA binding proteins are determined in the competitive experiment, and the oligonucleotide fragment (specific), and other non-associated fragments (non-specific), and other non-associated fragments (non-specific) to determine the specificity of DNA or RNA binding proteins. Specific binding is determined based on the characteristics and strength of the complex in the presence of competition. Sample preservation and transportation: protein and tissue samples - 80 ° C preservation, prevent protein degradation, 1-8 per film can be made Sample.

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