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JRD282 Immunuscularization (CO-IP) Method

Time:2019-09-07 Click:681

Immunocetallocalypse (CO-IP) Method Technical Services

Immunofucetochemical (CO-IP) Method Technical Services (CoImmunopRecipitation) is an in vitro A method of detecting whether there is a specific interaction between two protein molecules. The principle is if the two proteins can specifically interact with the in vitro system, and when an antibody of a protein is immunoprecipitated, the other protein will be simultaneously
. Unlike yeast double hybridization techniques, immunocytoprecipitation techniques are utilized by immune responses between antigens and antibodies, and is a method of researching protein and protein in vitro non-cellular environments.
The interaction proteins are modified after translation, in natural state; protein interaction is carried out in natural state, can avoid human influence; separation of the interaction protein complex of natural state.
Experimental steps:
1. u0026 nbsp Incubation antibody 2. add samples to protein A / g agarose resin 3. Centrifugal removal protein

u0026 nbsp 4. Elite binding protein

5. WesternBlot test or mass spectrometry



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