JRD337 real-time fluorescent quantitative PCR (TAQMAN probe method) technical service
Real-time fluorescent quantitative PCR (TAQMAN probe method) technology
real-time Fluorescence Quantitative PCR (TAQMAN Probe Act) The technical service avoids the conventional PCR to determine the deviation from the final product detection, and improve the repetability of the experiment. This technology has been widely used to detect changes in cell mRNA expression; compare differences in mRNA in different tissues or cells; experimental results of gene chips, siRNA interference. Bochuan Biology provides you with full range of fluorescent quantitative PCR technology services, all experiments are used in imported reagents and equipment: you only need to provide organizational or cell specimens, we have completed RNA extraction, cDNA preparation, primer design, quantitative PCR amplification , The steps of standard curve preparation and data analysis. Among them, TAQMAN probe method is a method of real-time fluorescence quantitative PCR.Real-time fluorescent quantitative PCR (Taqman probe method
) Serviceis an oligonucleotide probe, a fluorescent gene being attached to a 5 u0026 RSQUO; end, and the quenching group is in 3 u0026 # 39; the end. A pair of primers is added to the PCR reaction system, and a specific fluorescent probe is added. When the probe is complete, the fluorescent signal transmitted by the report group is extracted when the group absorbs the PCR amplification, 5 u0026 # 39 of the TAQ enzyme; 3 u0026 # 39; External enzyme activity disinteresses the probe enzyme to separate the report fluorescent group and the crushing fluorescent group, so that the fluorescent monitoring system can receive a fluorescent signal. Winning creatures provide high quality TAQMAN probe synthesis services and provide customers with relevant. If you want to know the company's real-time fluorescent quantitative PCR (Taqman probe method ) Technical service products or other products, consultation, we will warmly serve you.