JRD335 plasmid extraction technical service

Plasmid extraction technical service should pay attention to: 1, rock time: overhaura Culture is a universal acceptable concept and is suitable for most situations. If there is a problem, the adjustment culture time will help: NICK more, increase the culture time; the enzyme is problematic, and the culture time is reduced. 2 Every time you observe the habit of bacterial volume, because the plasmid is in the bacterium, and the amount of the extracted granules is only related to the amount of bacterial.

3, the suspension of the bacteria: If there is no suspension, the residual bacterial group is in solution II after adding, becomes one The peripheral cracking, no cracking in the middle, there is no cracking group in the middle. This group is in solution III after the addition, some protein continues to be present in the solution and becomes a large source of protein residues. 4, using a relatively excess reagent: this is a suggestion suitable for nucleic acid extraction. The relative excessive benefits of reagents is: good stability, high purity, simpler operation. If you think this is not economical, you will use less bacteria.

5, lysis time: Add solution II after mixing, the system can become clear immediately. If the system becomes clear, the solution is added to III III neutralization. If the system is not right, it is less useful next time, or more solutions. Today's plasmmets are more complicated, and weird phenomena also have more and more, and the strange phenomenon is related to the cleavage time.

6, a neutralization operation: Add solution III after the 1.5 ml centrifuge tube; then trumps twice, so that the bottom of the pipe Use the finger to pose a tube several times, then press down. The effect is very good.

7, the neutralization of centrifugal deprotein: centrifuge protein. If there is still a protein floating in the liquid after centrifugation, the effect of continuing centrifugation is not good; and pour the supernatant into another centrifuge tube, then centrifuge, the effect is much better.

AY Pharma was founded in 2005,The company is located in 7865 SW Nimbus Avenue (Space 29-G) Beaverton OR 97008, laboratory area of u200b u200bmore than 600 square meters. The company currently has better biology, medical technology service platform, which provides customers with medical research sample testing, cooperative research, development and production services. We have professional laboratories, advanced experimental equipment and experienced research technicians. Technical teams include two researchers (post-doctoral), 4 assistant researchers (Ph.D.), 15 core researchers (Master), mainly dedicated to genomics, transcriptional, proteomics, metabolic, biochemistry, pathological testing, Application and promotion of genetic testing in scientific research. Through highly subdivided, better service platforms, our customers provide comprehensive technical solutions and services. Currently, and, involving clinical medicine, tumor biology, immunology, cell biology, molecular biology, etc.

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