JRD281ITRAQ Method Technical Service
iTraq Hydrographic Services
ITRAQ Hydrography Technical Services of 4 isotopic labeling reagents from 4 The relative molecular mass of the reporting group of 114, 115, 116, and 117, a mass balance group of 31, 30, 29, and 28, respectively, respectively, respectively, respectively, respectively, and one of the same peptide reactive marker reagent base. PEPTIDE ReactiveGroup consists of. Different reporting groups respectively match the corresponding balance groups, relative molecular masss are 145, namely the isobaric tag. Cracking the protein into a peptide, and then labeled with ITRAQ reagents. Since the ITRAQ reagent is equal, the different isotopes are labeled in the * grade mass spectrometry after marking the same polypeptide, and the molecular mass spectrometry is used to detect the precursor ion (Precursorion) induced by * grade mass spectrometry. Analysis by the second stage mass spectrometry. During the secondary mass spectrometry, the bond barrier between the report group, the mass balance group and the polypeptide reactive group, the mass balance group lost, producing a low quality (M / Z) report ion. Since the secondary mass spectrometry can analyze the reporting group of the relative molecular mass difference 1, the difference in ion intensity of different reporting groups represents the relative abundance of the polypeptide it labeled. At the same time, the amide bonds in the polypeptide are broken, forming a series of B ions and Y ions to obtain the mass number of the ionic fragment, and can identify the corresponding protein precursors through the database query and comparison.
[ 123] Technical Features: 1. 2 to 8 samples can be marked simultaneously. 2. Itraq technology not only finds cytoplasmic proteins, also film proteins, nucleobers, and extracellular proteins. Itraq technology can detect proteins with low abundance proteins, strong alkaline proteins, less than 10 kD or greater than 200kd.
3. Marking the modified amino acid, thus conducting a qualitative quantitative study of the post-translation modified protein such as phosphorylated protein. 4. Relative molecular mass of the report ion is lower (113 to 121), and the low molecular weight zone is a region with mass spectrometry (quality determination) and quantitative (abundance comparison), so mass spectrometry is more sensitive and reliable.
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