TUNEL test experiment

Cell DNA is broken, deoxyribonucleoside TUNEL-mediated tunel Tuxel Tuxyl TUNEL DNA3 u0026 # 39; -OH is connected to biotin to biotin with biotin by the action of the deoxyribonucleotide terminal transferase (TDT), so that the fracture DNA is stained. Thereby analyzing apoptotic cells.

Apoptosis is late, chromosome DNA double strand breaks or single-strand break produces a large number of viscous 3-OH ends, and transfers at end of deoxyribonucleotide ( Under the action of TDT, the fluorescein / enzyme-labeled DUTP binds to the 3-end of DNA, so that the detection of apoptosis by detecting fluorescence, such a method is called deoxyribonucleotide terminal transferase mediated Terminal-deoxynucleotidyl transferase mediated nick end labeling, tunel) TUNEL detection experiment

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1. Cell climb 2. Tissue Slices

  3. Tissue    

1. Dyeing sample 2. Experimental results Photo 3. Experimental report [ 123]