JRD279SiLAC (quantitative proteomics)

SiLAC (quantitative proteomics) 1, SiLAC (quantitative protein group Principles: SiLAC ( Stable isotope labeling with) technology is a metabolic tag designed by proliferation of essential amino acids (Lys, Arg, etc.) using mammalian cell proliferation Method of protein. Take LYS as an example, there are 6 12C atoms in ordinary Lys molecules, and use 13C to replace all 12C atoms in ordinary Lys, thereby generating 13C marked Lys. The 13C marked Lys is more than 6DA than ordinary Lys.

For example, two groups of cells, a group of culture medium containing ordinary Lys (remembered K0), a group of Lys culture containing 13C (rejected K6). Since Lys is an essential amino acid of cell proliferation, the 13C-labeled Lys Natural u0026 ldquo; incorporates u0026 rDQUO; into the new and formed protein, the protein group of the entire cell is marked by the 13C labeled Lys. In view of the two amino acids of K0 and K6, there is a difference in mass of 6da, and when the protein is analyzed in subsequent LC-MS / MS, the identification and quantification of proteins in the sample can be completed simultaneously (6DA quality difference). According to the quantitative results, it is possible to directly determine the difference in protein expression, distinguishable specificity and non-specific interactions.

Second, SiLAC (quantitative proteomics) Features: (1 for cells protein interactions and quantitative proteomics technology of protein expression difference; (2) passed LC-MS / MS analysis, energy simultaneously achieves u0026 nbsp for protein molecules in unknown samples; Identification and quantification;
(3) According to quantitative results, it can directly determine the difference in protein expression, distinguishes and non-specific mutual Role.


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