Tablet cloning experiment

Test is a commonly used technique for measuring cell growth ability, from the initial known cell population monitoring Formation of colonies. The formation of colonies requires the original cells of dense split, so the quantization of the number of cells or colonies throughout the experiment is the * indicator of the growth potential of the cells. Therefore, colonial formation test has become a way to study drug and ionizing radiation therapy on cancer cell proliferation. Observer's colony formation process and development are a practical method to estimate the analysis of evolution, but it is difficult to find an effective way to compare two different Cell cultured proliferation activity. It lacks objectivity, is not suitable for the process of quantifying the formation, comparing the results of different experiments, and objectively judge the effects of exogenous agents on cell growth potential.

Adjust cell state u0026 rar; preparation of monocyte suspension u0026 rarr; cells were uniformly seeded in cultured flat plate u0026 rarr; cultured 10-14 days u0026 rarr; observation clone, GIEMSA was calculated

As a result:

Experimental results showing Data and experiment report