JRD275CHIP protein experimental technical service
AY Pharma was established in 2005, the company is located in 7865 SW Nimbus Avenue (Space 29-G) Beaverton OR 97008, Experiment The room area is 600 square meters. The company currently has better biology, medical technology service platform, which provides customers with medical research sample testing, cooperative research, development and production services.
CHIP protein experimental technical service operation procedure:
1 Sample preparation and cross-linking After collected, 37% formaldehyde treatment cells, Crosslinked nucleic acid and DNA binding protein;
2. Ultrasound interrupt quality
解 细, use bioruptor to break the chromatin ultrasound into 200-1000 bp pieces; divide the fragment into two copies, one Used in immunoassay, one for control.
3 , immunopetal precipitation (IP)
A fragment obtained from the B step was combined with CHIP specific antibody to carry out immunocutant, and the antibody complex was enriched by immunomagneamigh.
4 Decryption and Purification DNA
Inter-enriched DNA / protein complex interprets the release of DNA fragments and purified (IP DNA); B step obtained for control (Input) The fragment of the DNA does not perform IP experiments, but it is also to be interpreted and purified.
5 DNA Segment u0026 Nbsp; PCR Amplifies
Design specific primers for the target fragments of CHIP DNA, Input DNA, and control DNA and fluorescent quantitative PCR amplification.
6 Results Analysis
% Input u003d 2 (CTINPUT-CTCHIP) u0026 Times; FD u0026 Times;
fold enrichment u003d [% (CHIP / INPUT)] / [% (NEGATIVE Control / INPUT )
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