JRD274CHIP chromatin immunoassay precipitation technology service

CHIP chromatin immunocolite precipitation technology [ 123] It is true and completely reflected in the regulatory protein bonded to the DNA sequence, which is a well-known method that is currently determined in genomic regions that bind to a particular protein or a good method of determining proteins that bind to a particular genome region. The combined microarray technology is used to examine the staining activity in chromosome gene expression regulatory region, which is a very effective tool for analyzing the main metabolic pathways of cancer, cardiovascular disease, and central nervous system disorders. : The genomic DNA of eukaryotes exists in the form of chromatin, and the protein and DNA are stained. The interaction in the quality environment is the basic way to elucidate the expression mechanism of the true nuclear biological gene. Compared to conventional EMSA technology, chromatin immunoprecipitation technology (CHIP) can truly fully reflect the regulatory protein combined in the DNA sequence, which is a good method for studying the interaction between DNA and protein in vivo.

The basic principle is to fix the protein-DNA complex in the live cell state, and the ultrasound is disconnected to a chromatin fragment in the length range, and then the target protein antibody is added, and the target protein-DNA complex is obtained by antibody. The enriched target protein-DNA complex is obtained by elution method, and the fragment of the concentrated protein-binding DNA is particularly enriched, and the protein and DNA interaction are obtained by purification and detection (PCR analysis) of the target fragment. Information.

This technique is mainly used in: 1. Group protein modification research 2. Transcription regulation analysis 3. Drug development research 4. Miscal split study 5. DNA injury and apoptosis analysis. Basic steps:
1) formaldehyde treatment cell
2) collect cells, ultrasonic crush ] 3) Antibody to the destination protein, combined with the target protein-DNA complex 4) to add Proteina, bind to an antibody-target protein-DNA complex, precipitation
5) to clean the precipitated complex In addition, some non-specific binding 6) elution is obtained, resulting in an enriched target protein-DNA complex 7) to dissipate interception, purify the enrichment of DNA fragment 8) PCR or gene chip analysis . If you want to know other products, consultation, we will serve you.