JRD2682D-DIGE protein technical service

DiGe u0026 mdash; Two-Dimensional Fluorescence Difference Gel Electrophores, 2D-DIGE protein technology services [123 ] At the same time, the sensitivity of fluorescent dyes and the use of internal standards is applied, so that it is significantly better than traditional two-way electrophoresis in the study of quantitative proteomics.

The fluorescent dye used by DiGe has Cy2, CY3 and CY5, which can be labeled with the protein of lysine side chain amino groups, labeled proteins, etc. The electricity point and the molecular weight are not affected. After the equal amount of mixed protein, two-way electrophoresis, and the different gels can be matched by Cy2 internal standard, eliminating the difference between gels, and changes in protein expression via CY3 and CY5 The intensity of different fluorescence is reflected. (123)
Second, DiGe has the following advantages compared to conventional two-way electrophoresis, silver dye or insulting adhesive:
1, high sensitivity, low can detect protein;
2, high-efficiency, the same gel can electrophore 2 Sample, mitigating a workload;
3, the linear range is wider, the dynamic range is up to 10-5;
4, quantification, using internal standard eliminating experimental errors between gels and gels, significantly increased the degree and reproducibility of experiments;
5, Statistical analysis, ImageMaster7.0 (DiGe) software can obtain statistically trusted results and reduce the deviation between operators.
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