JRD267SiLAC protein technical service

Background introduction:

[ 123] SiLAC protein technology service (labeling with ") technology is based on cell growth for the dependent principle of essential amino acid, using stable isotope labeled (13C, 15N, 2H) The essential amino acid is a method of metabolizing the cellular protein group;

Arg (r), Lys (k), and Leu (L) are commonly used in SiLAC. R, K, L of (13C, 15N, 2H), such as R, K, and L is added to the cell culture medium, and when the cell is synthesized, these stable isotope labeled amino acids are naturally u0026 ldquo; incorporate u0026 rDQUO; into the protein. Through u0026 gt; 6 generation of cell proliferation, the protein group of the entire cell is stabilized with isotope amino acid u0026 rdquo; marker u0026 rdquo ;;

u0026 ldquo; marker u0026 rdquo; protein With u0026 ldquo; the number of proteins (normal medium culture) of unmissive u0026 rdquo; this quality difference exhibits a pair of bispressor peaks in a subsequent LC-MS primary mass spectrometry (MSL), by the signal strength of the mass spectrum peak The relative quantification of each protein expression level in the 2 group samples can be achieved, Finance to determine protein expression level difference (differential proteomics analysis) and distinctive specificity and non-specific interaction (protein) Analysis of interaction); simultaneous pass

The secondary mass spectrometry (MS / MS, MS2) is realized to identify the protein in the sample.

Techniques Combined with LC-MS can simultaneously realize the qualitative and quantitative analysis of proteins in 2 groups. Experimental technology u0026 ldquo; high, large, upper u0026 ldquo; quantification, passing through the ink information can directly reflect the difference in protein expression between the samples, easy to quickly find the target molecule of interest. At the same time, using SiLAC technology, it can significantly improve the grade. SiLAC amino acid control table:

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SiLAC experiment design:

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