JRD266TMT protein technical service

TMT protein technical services

TMT protein technical services are the same, and the molecular structure of the two is different. Compared to SiLAC (labeled protein), ITRAQ and were labeled with peptides. According to the mass difference between the ITRAQ / TMT tag, the protein in the multi-group sample is performed by MS to perform relative quantification of the protein in multiple sets: SiLAC is quantified by primary MS (MSL), and ITRAQ / TMT is a quantitative ITRAQ /
to compatibility with secondary MS (MS / MS.ms2), and can simultaneously analyze samples of 8-10 groups, analyze high. 1, extracting proteins and quantifies;

2, Trypsin performs enzymatic and hydrolysis of proteins, and extracts peptide segment; [ 123] 3, using different mass of ITRAQ / TMT tags, the peptide samples are respectively labeled:
After the marking is completed, the sample is mixed, and the component separation is performed with SCX (15-20 groups. Points); 5, LC-MS analysis for each component:
6, protein identification and quantitative analysis, screening differentially expressed protein; [123 ]
7, Biology Experiment Verification (Western Blot, EUSA, etc.): Technical advantages
1, high flux, can also perform up to 8 -10 set of qualitative and quantitative analysis of the sample protein group; 2, compatible sample (animal and plant tissue, bacteria, blood, etc.); 3, short experiment cycle.
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