JRD265Label-Free protein technical service

Label-Free Protein Technical Services : Non-standardized proteomics technology

For example, you have 10 tissue samples, extracting proteins and enzymatically resolved into peptide segments. Each peptide sample was then analyzed to obtain 10 MS data;

For 1 peptide, its abundance in 10 samples (amount) In addition to the peak area or signal strength in MS1;

Theoretically, for the same peptide segment in 10 samples, the retention time on the LC (Retention Time) RT) is *, therefore, the quantitative analysis of the relative expression levels of the protein can be achieved by comparing the MS map.

1, compared with SiLAC, ITRAQ / TMT technology, the sample does not need to be marked, and the sample can end the sample. u0026 ldquo; Authenticity u0026 Rdquo ;;

2, the sample processing step is simple, and LC-MS analysis can be performed by only the enzymatic solution;

[123 ] 3, can save a lot of sample preparation costs.

Maxquant, ProGENESIS, etc.

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