Cell co-culture experiment
In the late 1980s, in order to establish a cultivation system that is more similar to in vivo environments, in vitro environments with the in vivo environment, Thus, the cells can communicate with each other, support each other, and the development of cell co-culture techniques are developed based on cell culture technology. Cell co-culture techniques are cultured in the same environment in two or more cells, since they have the advantage of better reflecting in vivo environments, so this approach is widely used in modern cell studies. Currently, cell co-culture techniques are mostly used in osteocytes and nerve cells. The cell co-culture system is mainly established by two methods: 1 Direct co-culture system, which is more than two or more cells or inoculated in the same well, and the different kinds of cells are directly in contact; 2 indirect co-culture system, Two or more cells are inoculated on different carriers, and then the two carriers are placed in the same culture environment, and different types of cells are shared in the same culture system without direct contact.
The main role of co-culture system: induces cells to differentiate to another; induce cells themselves; maintain cell function and vitality; regulate cell proliferation; promote early embryo development and improve metabolites production.
Cell co-culture experiment
[123 ] Cell strains (freeze-containing strains or cultured cells).
Basic experimental steps, pictures, data analysis results.
Experimental period: 2-3 weeks, specifically, according to cell growth and experimental content.