Mitochondrial film potential (MMP) test experiment

Mitochondrial Film Potential (MMP) Test Experiment MMP (Mitochondrial Membrane Potential) is a mitochondrial membrane potential, and mitochondria is the main location of animal cells to generate ATP, which is to promote cell energy conversion. An important orpatter participating in apoptosis.
Mitochondrial film potential (MMP) test experiment

1. Cell culture; 2. Induce apoptosis with an appropriate method while establishing negative according to combined positive The control group was collected by the cells; 3. Wash the cells three times with PBS, collecting no more than 1 u0026 TIMES; 106; [123

4. Take 100 u0026 Mu; L 10 u0026 Times; Incubation Buffer plus 900 u0026 mu; l Sterilization deionized water to 1 u0026 Times; Incubation Buffer, mixed Energe and preheating to 37 ° C; 5. Paints 500 u0026 MU; L 1 u0026 Times; Incubation Buffer, Join 1 u0026 Mu; L JC- 1, vortex mixing into JC-1 working fluid;

6. Take 500 u0026 mu; L JC-1 Working The cells were evenly suspended, and incubated in an incubator of 37 ° C, 5% CO2 was incubated for 15 to 20 min. 7. Room temperature centrifugation (2000 rpm, 5min) collection cells, washed twice with 1 u0026 Times; Incubation Buffer;

8. Paints 500 u0026 Mu; L 10 u0026 Times; INCUBATION BUFFER resuspension cells;

9. Flow cytometry testing, analysis. Mitochondrial film potential (MMP) test experiment

Cell strain (freeze-containing strain or cultured cell), related drugs or MMP detection reagentsBox (available) Company provides

Experimental period: 1-2 weeks, specific needsDepending on cell growth and experimental content.