Cell migration experiment

Cell migration experiment

Cell migration and invasion experiments put the Transwell chamber into the culture plate, the interior is called the chamber, and the cover is referred to in the culture plate, the upper and lower layers The culture solution is separated by a polycarbonate film, and the cell species of the study will be in the upper chamber. Since the polycarbonate film has permeability, the ingredients in the lower culture solution can affect the cells in the upper chamber, and different apertures and different treatments are used. The polycarbonate film can be studied in co-culture, cell chemokine, cell migration, cell invasion, and other aspects. Cell migration experiment

Experiment

1. Uniform line with a Marker pen on a 6-well plate; [123 2. Add cells in the well;

3. The next day, the gun head is draped to the horizontal scratches behind 4. Wash the cells with PBS, Different times, sampling, photographing;

5. Data processing: Each time point cell overall migration distance u003d Each time point Distance length -0 distance; 6. Result statistical analysis.

TRANSWELL experiment

1. Transwell chambers that were not paved in MATRIGEL glue were used for experiments; 2. Preparation of cell suspensions; [123

3. Injection of cells; 4. The number of cells passed;

5. Results statistical analysis. [12

Cell strain (frozen storage or cultured cell),The drug, drug treatment method and concentration.

Company provides

Basic experimental steps, pictures, data analysis results, surplus drugs.

Experimental cycle: about 2-3 weeks, specifically, according to cell growth and experimental content.