Screening experiment of stabilizing cell line

Stabilized cell line, generally cloned exogenous DNA To a vector having a certain resistance or selective gene, the carrier is transfected into the host cell and integrated into the chromosome, and screens the selection mark contained in the carrier, and screens the cell line that can stably express the protein.

[ 123] The expression of foreign genes can be divided into two major categories, one is instantaneous expression, and one is stable expression (also called * expression). The former exogenous DNA / RNA is not integrated into the host chromosome, although high levels of expression can be reached, but usually only for a few days. The latter exogenous DNA is integrated into the host cell chromosome, allowing the host cell to express the genetic gene. A stable cell line is established, typically screened with the target cells based on resistance marks contained in different gene carriers. Commonly used resistance marker genes have hygromycin, neomromycin and puromycin.

Experimental process

1, vector construction (expression plasmid vector, viral vector);

2, transfection / infection;

3, the preliminary detection of the object of interest;

4 , Antibiotic screening;

5, the protein of interest (qualitative / half quantitative);

6, the stability of monoclonal cell lines.

Customers provide

Cultured cells Five, the company provides:

[123 ] Basic experimental steps, pictures, data analysis results and stable expression of cell line

Experimental cycle: about 3-4 months, specific needsDepending on the cell experiment.