Cell proliferation experiment
Cell proliferation experiments Cell proliferation is a basis for organism growth, development, breeding, and genetic basis, and is a basic physiological activity of cells. Growth factors, hormones and cancer products, etc., can induce a proliferation of cells or inhibit the proliferation of cells, and achieve cellular apoptosis by affecting cell cycle.
MTT is all called 3- (4, 5) -Dimethylthiahiazo (-Z-Y1) -3, 5-di-PhenyTetrazoliumromide. The MTT colorimetric method is a commonly used method of detecting cell survival and growth. Its detection principle is that the succinate dehydrogenase in the living cell mitochondrial can reduce the exogenous MTT to be water-free blue purple crystal broth and deposit In the cell, there is no such function. The dimethyl sulfoxide can dissolve the acetrane, and the light absorption value is measured at a wavelength of 490 nm with a microplate apparatus, and the amount of the crystal formation of the crystallization is proportional to the cells in the range of 490 nm. The absorbance value can indirectly reflect the number of live cells. This method has been widely used in anti-tumor drug screening, cytotoxic test, and tumor radiological sensitivity assay.
Cell Counting Kit-8 (referred to as CCK-8 ) reagents can be used for simple and accurate Cell proliferation and toxicity analysis. Its
Basic Principleis: The reagent contains WST-8 [chemical name: 2- (2-methoxy-4-nitrophenyl) -3- (4-nitrophenyl) - 5- (2,4-disulfonate) -2H-tetrazole single sodium salt], in electron carrier 1-methoxy-5-methylpheniazinate dimethyl sulphate (1-Methoxy PMS) The dehydrogenase in the cells was reduced by the dehydrogenase of the cells with a highly water-soluble yellow nail product (Formazan Dye). The number of methyls produced is proportional to the number of live cells. Therefore, this characteristic can be utilized to directly conduct cell proliferation and toxic analysis.