Hangzhou cell scratches experiment

Hangzhou cell scratches experiment.

(Wound Healing) is a simple operation of studying cell migration capabilities. The principle is that when the cells grow to a single layer state, people in the fused single-layer cells are produced a blank area, which is u0026 ldquo; scratches u0026 rdquo;, the cells of the scratch edge will gradually enter the blank area to make u0026 ldquo; Scratch u0026 rdquo; hea. The migration capabilities of cells were judged by observing the status of cellular cells in different periods.

Note:

Generally, scratch experiments are cultured in serum-free or late blood, otherwise cell proliferation cannot be ignored. According to the vertical direction of the drawing line behind the 6-well plate, a number of intersections can be formed as a fixed detection point. The problem of not being fixed when the position before and after observation is solved.

1. First use the Marker pen behind the 6-well plate, The size is more, evenly scratched, about 0.5 to 1 cm, cross the hole horizontally. Each hole passes at least 5 lines.

2. About 5 x 105 cells were added in the air, and the specific quantity varies depending on the cells, and the mastery is overnight. 3. The next day with the gun head ratio has a straight foot, try to be slightly scratched behind, the gun head is vertical and cannot be tilted. 4. With PBS wash cells 3 times, the cells were placed, and serum-free medium was added.
5. Put 37 degree 5% CO2 incubator, cultured. Press 0, 6, 12, 24 hours to sample, take pictures.
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