In situ hybridization experiment

In situ nucleic acid molecules hybridization technology referred to as in situ hybridization, using nucleic acid The base complementarity between the molecular single-strand is paired by the principle of forming a double-stranded hybrid molecule, and DNA or RNA is tested on the tissue, cells or chromosomes, and then display the probe. CRNA (complementary) probe was detected by CRNA (complementary) probe in situ in situ. Under the conditions of the cell or tissue structure, the known RNA nucleotide fragment is used in the nucleic acid hybridization, and the corresponding gene fragment in the cell or tissue is combined with the corresponding gene fragment to be tested (hybridization) in the cells. The corresponding mRNA molecules in its cells were observed under optical microscopy or electron microscopy after developing, mRNA can be used as a negative control.

In situ hybridization experiment service content:

1. mRNA in situ hybridization Preparation and detection of

(1) mRNA antisense probe design;

(2) Probe primer design;

(3) mRNA antisense probe synthesis,

[123 ] (4) MRNA antisense probe quality detection, positive and negative slice each,;

(5) Slice in situ hybridization detection MRNA expression.

(1) in situ hybridization probe sequence and sequencing map

(2) In situ hybridization probe concentration measurement results;

( 3) Probe detection, positive and negative photos;

(4) in situ hybridization detection destination mRNA, positive and negative control results slice;

(5) In situ hybrid sliced u200b u200bmicrograph. Nbsp;