JRD351 steady transform cell line construction technology services
Introduction: low toxic, high transfection efficiency liposome transfection is suitable for ad hoc cells Transfection with suspension cells; through liposome-mediated transfection, the purpose gene is introduced into cells for 48 hours, and the gene is expressed in intracellular. At this time, in order to establish a stabilized cell line, the target cell can be screened according to the resistance marker contained in the different gene carriers, thereby obtaining a cell clone that stably expressing the exogenous gene.
Provides reliable, universities of steady cell line construction services, with many years of steady transcendal cell line construction technology services experience.
Low toxicity, high-transfection efficiency liposome transfection is suitable for transfection of wall cells and suspended cells; through liposome-mediated transfection, the purpose gene is introduced into cells for 48 hours, and the purpose gene That is, in intracellular expression. At this time, in order to establish a stabilized cell line, the target cell can be screened according to the resistance marker contained in the different gene carriers, thereby obtaining a cell clone that stably expressing the exogenous gene.
Service Flow 1) Screening Concentration Determination: Antibiotic concentration of all deaths before the previous day is a screening concentration 2) Cell inoculation: transfection The cells were inoculated before the experiment (the laying density was different from the species of the cells), so that the transfection was 80% of the cell transfection 3) cell transfection
4) Antibiotic screening
5) Identification screening results
Turnocyte strain constructs
1. A plasmid which can be directly used for transfection
3. About cell culture conditions Note: The company provides DMEM medium, RPIM1640 medium, puromycin and G418 antibiotics, such as other medium and screening conditions, please also provide customers. Special genes Please provide the corresponding antibody to detect
We provide to construct a good stable cell line, experimental report Quality Transfection cells exogenous gene stable expression [123
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