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JRD322 electron microscopy technical service

Time:2020-03-23 Click:325

Electron microscope detection technical service is a product of immunochemical techniques and electron microscopy techniques, and is a method for studying and observing antigen, antibody binding positioning in ultrastructural levels. Currently common immuno electron microscopy technology is divided into embedded immune electron microscopy technology and embedded immune technology, and the embedded immuno electron microscope technology is the first in thin specimens with a diameter of less than 1.4 NM immunotropic labeling destination protein, silver After the enhancement signal is enhanced, the position of the purpose protein is observed with conventional electron mirrors. After the embedded immunochroniography, the test specimen is first cut into ultra-thin slices, and then single or multi-marking on the ultra-thin slice with different diameters of immunogels, to illustrate the location and mutual mutual number of proteins in ultrastructure. Role relationship. Electron microscope detection technology service operation procedure:
Bulcted immuno electron microscope procedure: Sample fixation one rinsing one-to-one anti-marking immunization gold two standard marker 1 silver reagent to enlarge a conventional electron microscope fixed, dehydrated, embedding, slice , Dyeing, observation.
After embedded immunization electron microscopy: sample fixes a drifting / dewatering one K4M or L.R. WHITE embedded a ultra-thin slice one-one anti-marking an immune gold label II marking a conventional electron microscope stained, observation.
Sending sample requirements:
Signing a service contract, providing the corresponding one-to-action.
Service Contract Terms:
1. Samples are fresh, and the fixing method is strictly in accordance with the experimental steps we provide.
2. Provides the corresponding one-resistance and provide a dyed image under the mirror.
3. When we do immune electrical mirror, we will set the fluorescent label of the lens as a control. If the control has a positive reaction, the result of the immuno electron microscope is negative, we will not charge the cost of immuno electron microscopy, If the control is negative, we will charge in accordance with normal costs. At the same time, we will also provide a conventional electron microscope structure as a control. If the super-microstructure is abnormal, we will not be able to immunize the quality of electron microscopic images.
4. Ultrastructural images of immuno electron microstructure than conventional electron mirror, as long as we can identify ultrastructure images, there is a significant marker.
5. Report time: Experimental reports in 2 months (from samples), if you need to get results within one month, you need to add 20% service charge.

Currently, Shanghai AY, Main Experimental Technical Services, welcomes the majority of research enthusiasts to come to consult.