JRD320 slow virus packaging molecular experiment

Characteristics:

[123 ] 1. Direct packaging into fake viral particles, infected with split and non-split cells, suitable for RNAi studies and cells that are difficult to transfection in experiments in vivo (such as neuronal cells, stem cells or other primary cells).

2. A variety of cell lines expressing a particular gene can be obtained in a short period of time.

3. Study available for gene knockout, gene therapy and transgenic animal.

4. Easy to operate without transfection reagents.

5. A variety of tags can be prepared according to customer needs.

Lentivirus is a kind of reverse transcriptional virus. The constructed siRNA / miRNA lentiviral vector, with a chemically synthesized siRNA and a normal siRNA vector constructed by the instantaneous expression vector, on the one hand, an inherction, an in-one replacement instantaneous expression vector, on the other hand, Lentivirus-siRNA cloning passes through a slow virus package After the system is packaged, it can be used to infect cell lines that rely on traditional transfection reagents, such as primary cells, suspended cells, and non-fractional cells, and can be integrated into the genome of infected cells after infection, for a long time Stable expression.

1. Construction of a slow viral expression vector containing a target gene Purification of plasmid purification. Note: The customer can also provide a constructive recombinant lentiviral expression vector.

2. Slow virus expression vector and packaging system co-transfected viral packaging cells.

3. Collect viral fluid.

4. Purification and concentrated viruses.

5. Save, - 80 OC. 6. Titness assay and sterile detection.

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