JRD254 gel migration protein technical service
Gel migration protein technology services Introduction: Gel migration or electrophoretic mobility experiment is a study of DNA binding proteins And the technology of its associated DNA binding sequence can be used for qualitative and quantitative analysis. This technique is initially used to study DNA binding proteins, which have been used to study the interaction of RNA binding proteins and specific RNA sequences.
Generally, purified proteins and cell coarse extracts and 32P isotope labeled DNA or RNA probes are typically thermally insulated. Divided polypropylene gel electrophoresis, separating complexes and non-binding probes. DNA-composites or RNA-composites are slower than non-binding probes. The isotope labeled probe is different from the binding protein of the study, but the double-strand or a single strand. When detecting DNA binding proteins such as transcriptional regulators, purified proteins, partially purified proteins, or nuclear cytokines can be used. When the RNA binding protein is detected, the protein can be extracted or partially purified according to the position of the RNA binding protein. The fragment of DNA or RNA containing protein binding sequence and the oligonucleotide fragment (specific), and other non-associated fragments (specific) in . Non-specific) to determine the specificity of DNA or RNA binding proteins. Specific binding is determined based on the characteristics and strength of the complex in the presence of competition. AY Pharma, company located in 7865 SW Nimbus Avenue (Space 29-G) Beaverton OR 97008, comparable to Shanghai Fudan University 600 square meters in the laboratory area. Currently, there is an animal experiment center, pathological experiment platform, cell experiment platform, molecular experiment platform, with better biology,