EDU cell proliferation test
1. Cell culture: Take the number of growth cells, inoculated in 96-well plates in 96-well plates, 4x103 to 1x 105 cells per well, cultured to normalGrowth phase
2. Drug treatment: Various pharmaceutical treatment according to experimental needs
3. EDU mark: The EDU solution (reagent A) is diluted with cell culture medium (reagent A),Preparation of a child 50 u0026 mu; M EDU medium;
Note: 1) EDU concentration is related to incubation time, short time incubation ( u0026 lt; 2h should adopt high concentration (10 ~ 50 u0026 mu; m); long timeIncubation ( u003d 24h) should be used to use low concentration (1 to 10 u0026 mu; m);
a. If you need to configure 10 u0026 MU; M EDU medium, you need to adjust to 5000: 1 Dilution ratio
BThe preservation time of the configuration of the medium depends on the properties of the medium.
5. Apollo dyeing
6. Image acquisition and analysis [123
Customers provide1. Provide fresh heparin or EDTA anticoagulating peripheral blood
2. Provide fresh cultured cells, provide fresh tissue, and is a single cell.Suspension. We offer
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