JRD521 primary cell separation experiment
Inproduction Cell Isolation Experiment
Cell cultures refer to the culture from the body to remove cells, tissues and organs. Therefore, it is more strictly to refer to the cultivation of successful passage. At this time, the cells maintain the basic properties of the original cells, and if it is normal cell, it is still reserved that the * generation to the tenth generation of cultured cells Recently referred to as primary cell culture.cultivated organic block culture with commonly used primary cells (tissue block culture is directly transplanting the cracked tissue block in the culture bottle On the wall, the culture medium was added and cultured.) Dispersing cell culture (dispersion cell culture is substantially step in which the animal tissue removes from the body to a single cell, common trypsin is dispersed, and the appropriate medium is cultured. The cells are survive, grow and breed). Separation method of suspension cells commonly used in primary cell separation experiments, and the separation method of solid tissue materials.
Category
[123 2, bone marrow mesenchymal stem cells (BMSCs)
[123 ] 3, brown adipocyte[ESCs)
5, glomerular endothelial cells
6, uterine fibroid cell
8, Other
Applications include: cardioma fibroblasts, rat cartine cells, human ovarian cancer primary cells, colon epithelial cells, etc.
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