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JRD526iHC immunohistochemical pathological service

Time:2018-05-01 Click:396

IHC immunohistochemical pathological services

[ 123] Tissue and cells of tissue sections or cell specimens in tissue slices or cell specimens, referred to as immunohistochemistry, technologies or immunocytes Immunocytochemistry technology. The immunohistochemistry is characterized by strong specificity, accurate positioning, and thus it can be clearly related to cell positioning, subcellular positioning and multi-protein between the protein. Immunohistochemistry is widely used in medical applications, especially in clinical tumor diagnosis and identification diagnosis. It is applied to a differential diagnosis of low dividing or undifferentiated tumors, and the accuracy can reach 50% -75%. Second,

IHC immunohistochemical pathological services

Experimental principle [ 123] According to antigen antibody reactions and chemical color development principles, antigenic and primary antibody binding in tissue sections or cell specimens, then react with secondary anti-labeled biotin, fluorescein or the like, The chemical component of the cell or tissue is displayed by coloring reaction or fluorescence, and the antigen antibody reaction product that occurs in the cell can be clearly seen under an optical microscope or a fluorescent microscope, thereby determining certain sites on the cell climb or tissue slice. The distribution and content of chemical composition.


Production of paraffin slices;
dewaxed wax slice With hydration;
block endogenous peroxidase activity; antigen repair; closed non-specific protein; an anti-incubation; has biotin-labeled second anti-incubation

SP (streptavidin-peroxidase) reaction;

DAB developed; reset, dehydrated, transparent, and seal. Mirror check, take pictures.


Fourth,

IHC immunohistochemical detection

customer provides


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5, IHC immunohistochemical detection [123 ] Company provides Experimental steps, result picture, results analysis.

Experiment cycle: 1-2 weeks.