ROS active oxygen detection experiment

Reactive Oxygen Species, ROSs include superoxide radicals, hydrogen peroxide, and downstream product peroxide and hydroxylate, etc., involved in cell growth proliferation, developmental differentiation, aging and Dear and many physiological and pathological processes. The DHE active oxygen detection kit is an active oxygen detection using a fluorescent probe DHE (Di Hydroethidium), and DHE can be free to enter the cell membrane to the cell membrane and is oxidized by intracellular ROS. Acetidine; oxycetidine can be incorporated into chromosome DNA to produce red fluorescence.

In the vicinity of the excitation wavelength of 535 nm, the emission wavelength is 610 nm, fluorescence is detected using a fluorescent microscope, a laser ammeter, a fluoresceransmetric microscope, a fluorescent spectrophotometer, a fluorescent enzyme, a flow cytometry, or the like, thereby determining cells Internal active oxygen level.

DHE probe marks:

1, DHE solution can be diluted in fresh culture, buffered saline, or tissue perfusion fluid to the desired concentration, 100-1000 times dilution, to stain The liquid is replaced with cell culture fluid or perfusion; or directly adding the DHE probe solution to the desired concentration directly to the cell incubation liquid or perfusion.

2, according to the difference in cell ROS, the DHE final concentration can be selected from the range of 100-1000 times dilution, incubation

time can be selected for 10-90 minutes, 37C or room temperature Anti-light incubation.

3, cleaning cells or tissue with fresh solution after the incubation.