Cell scratches experimental test

Ultra-clean workbench, CO2 incubator, biological inverted microscope, 6-well plate (other can, but feel 6 holes, size, size Moderately), Marker pen, straight ruler 20, microlifier head (sterilization), serum-free medium, PBS

Cell scratches

All can sterilize Sterilization, ruler and Marker pen in UV irradiation in the operation of 30 min (over closed station)

Experimental process

1. Behind the Marker pen on the 6-well plate, with straight The horizontal line is evenly scratched, and the hole is passed through every 0.5 ~ 1cm - Tao. Each hole passes at least 5 lines.

2. About 5 x 105 cells were added in the air, and the specific quantity was different due to cells, and the mastery was overnight.

3. The next day with a gun head is compared to a straight foot, so as to be tied to the horizontal scratches behind, the gun head is vertical and cannot be tilted.

4. With PBS wash cells 3 times, the cells were placed, and serum-free medium was added.

5. Put 37 degree 5% CO2 incubator, cultured. Press 0, 6, 12, 24 hours to sample, take pictures.

Cell scratches experimental service content and method

1, using the Marker pen behind the 6-well plate, evenly spread horizontally, about 0.5-1 cm, cross, cross hole . Each hole passes at least 5 lines.

2, about 5 * 105 cells are added to the holes, and the specific quantity is different due to cells, and the mastery is overnight.

3, the next day with the gun head is compared to the straight level, so as to be tied to the horizontal scratches behind, the gun head is vertical and cannot be tilted.

4, use PBS to wash cells 3 times, removed cells, and serum-free medium.

5, put in 37 degrees 5% CO2 incubator, cultured. Press 0, 6, 12, 24 hours to sample, take pictures.